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M9480842.TXT
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1994-09-05
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Document 0842
DOCN M9480842
TI Evaluation of a direct ELISA HIV-1/HIV-2 EIA using synthetic peptides:
detect-HIV (version 2).
DT 9410
AU Chalifour RJ; Ha NH; Barbeau S; Lacroix M; BioChem ImmunoSystems Inc.,
Montreal, Que., Canada.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:554 (abstract no. C-362).
Unique Identifier : AIDSLINE ASM94/94313103
AB A direct ELISA version of BioChem ImmunoSystems' synthetic peptide EIA
was evaluated to establish if detecting all immunoglobulin classes
improves the sensitivity of this test. HRP conjugates of 3 peptides from
HIV-1 GP120, HIV-1 GP41 and HIV-2 GP36 were used to detect antibodies
captured by these peptides on microtiter plates. Pending confirmatory
testing, the specificity of this EIA was 99.5% (1974 fresh samples
tested) while the sensitivity was 100% for HIV-1 (50/50) and 100% for
HIV-2 (15/15). By testing serially diluted samples the sensitivity of
the direct EIA was found to surpass that of the indirect EIA (version 1)
with seroconversion samples and was of similar sensitivity with samples
from later stages of HIV infection. The signal of both the direct and
indirect EIAs was reduced by > 90% after IgG absorption by Protein G
except with seroconversion samples where only a 30% signal reduction
occurred with the direct EIA. This indicated that the improved
sensitivity of the direct EIA with samples taken during seroconversion
is because the direct EIA format detects IgG as well as other
immunoglobulin classes.
DE Enzyme-Linked Immunosorbent Assay/*METHODS Human HIV Antibodies/BLOOD
HIV Envelope Protein gp120/*ANALYSIS HIV Envelope Protein
gp41/*ANALYSIS HIV Seropositivity/*DIAGNOSIS HIV-1/*ISOLATION & PURIF
HIV-2/*ISOLATION & PURIF IgG/BLOOD Peptides/CHEMICAL SYNTHESIS
Sensitivity and Specificity Viral Envelope Proteins/*ANALYSIS MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).